Conclusions and Perspectives on Plant Micropropagation in Temporary Immersion Systems.

In vitro propagation protocols that include temporary immersion systems are available for the most economically important plant species. However, these have not been established yet for multiple species. Having protocols validated by the scientific community guarantees the success of the mass production of commercial propagules. Besides, adequate TIS parameters should be established for each plant species to improve the efficiency of micropropagation processes. This book compiles basic and applied aspects of temporal immersion systems used for in vitro plant micropropagation, along with several detailed protocols already established, which may be used as a guide by those interested in this technique, including laboratory technicians, scientists, and other professionals.

Building pathology capacity in sub-Saharan Africa to improve breast cancer diagnosis and treatment: training laboratory technicians in high-quality manual immunohistochemistry.

BACKGROUND: To address the need for a skilled workforce in breast cancer (BC) pathology in sub-Saharan Africa (SSA), we implemented an education program to train laboratory technicians in manual immunohistochemistry (IHC). METHODS: A quality improvement education project was developed. Interactive webinars were held every six months with didactics and presentations from African experts with experience in IHC. We conducted knowledge assessments and surveys on current practice, equipment, and human resources. A digital mentorship platform (DMP) was created for discussions, sharing SOPs, and networking. For one year (2022-2023), we followed developments in pathology capacity, practice changes, and educational needs. A paired t-test was used to calculate the significance of changes in knowledge immediately after the webinar and comfort level with topics 35 days after the webinar. RESULTS: Two hundred and sixty six participants from 10 SSA countries attended the first webinar, a series of six lectures on IHC theory, methods, and practice. Ninety-five participants from nine SSA countries provided a baseline assessment of pathology capacity and feedback. Mean knowledge increased by 17.4% immediately after the webinar (from 41.8% pre-webinar to 59.2% post, p = < 0.0001). Self-reported comfort level in topics 35 days after the webinar increased by 11.3%, but this was not statistically significant (mean 3.36 pre- to 3.74 post, p = 0.1). Over six months, recordings were accessed 412 times. After six months, the second webinar had 93 participants from eight SSA countries. Membership in the DMP increased from 64 to 172; recordings were viewed 412 times in six months; and 113 participants from nine SSA countries completed surveys. Among 74 respondents who perform IHC, 43.5% reported moderate or significant positive practice changes such as improved antigen retrieval techniques and optimization of preanalytical variables. Over half (52.7%, n = 39) reported the quality of slides had moderately or significantly improved. After one year, a third webinar had 98 participants from eight SSA countries. Thirty-eight completed surveys, DMP membership increased to 199, and 1 reported launching IHC in a lab in Nigeria. CONCLUSIONS: Our program 1) reached hundreds of participants and provided a baseline assessment of pathology capacity across nine SSA countries; 2) created a novel mechanism to build pathology capacity and assess progress with this cohort; and 3) improved practices and the preparation of slides for over half performing manual IHC. After one year, interest was sustained. Tracking impact on diagnosis and treatment of BC in the region is needed long-term.

Interrelationship between dental clinicians and laboratory technicians: a qualitative study.

BACKGROUND: Effective communication among members of the dental team is essential for the delivery of high-quality dental care. However, an in-depth understanding of issues concerning the interrelationship between dental clinicians and laboratory technicians has not been previously undertaken. Therefore, the aim of the study was to explore factors influencing the interrelationship between dental clinicians and laboratory technicians. METHODS: Semi-structured interviews were conducted with dental clinicians and laboratory technicians using purposeful snowball sampling. Two trained researchers conducted the interviews based on a pre-piloted topic guide. The interviews were conducted via video conferencing platform, audio-recorded, and transcribed verbatim. Field notes were taken during the interviews. Framework Methodology was used to analyze the data. RESULTS: A total of 20 dental clinicians and laboratory technicians were interviewed. The average interview duration was 37 min. Participants mainly reported negative encounters and highlighted the importance of training and exposure, collaborative learning, and alignment of expectations of both parties in terms of cost of laboratory work, turnaround time, and patient preferences. The relationship between dental clinicians and laboratory technicians depends largely on effective teamwork dynamics and open communication channels. Increased workload, workforce shortage, availability of digital systems, management policies, and financial challenges were emphasized as organizational factors affecting the interrelationship between both groups. Participants highlighted the importance of shadowing, mentorship, education courses, joint discussions, patient-technician rapport, and adoption of digital technology for fostering collaborative practices between the professions. CONCLUSIONS: A multitude of factors influencing the dental clinician-laboratory interrelationship at individual, interpersonal and organizational levels were identified. This study highlights the need to build a transformative relationship underpinned by mutual trust and respect. Such a collaborative relationship will facilitate optimal patient care and successful treatment outcomes. The outcome of this study can help stakeholders identify solutions for enhancing the interrelationship among the dental team, to ultimately improve patient care and efficiency of dental services.

The roles and involvement of global health partners in the health workforce: an exploratory analysis.

Development partners and global health initiatives are important actors in financing health systems in many countries. Despite the importance of the health workforce to the attainment of global health targets, the contribution of global health initiatives to health workforce strengthening is unclear. A 2020 milestone in the Global Strategy on Human Resources for Health is that "all bilateral and multilateral agencies have participated in efforts to strengthen health workforce assessments and information exchange in countries." This milestone exists to encourage strategic investments in the health workforce that are evidence-based and incorporate a health labour market approach as an indication of policy comprehensiveness. To assess progress against this milestone, we reviewed the activities of 23 organizations (11 multilaterals and 12 bilaterals) which provide financial and technical assistance to countries for human resources for health, by mapping grey and peer-reviewed literature published between 2016 and 2021. The Global Strategy states that health workforce assessment involves a "deliberate strategy and accountability mechanisms on how specific programming contributes to health workforce capacity-building efforts" and avoids health labour market distortions. Health workforce investments are widely recognized as essential for the achievement of global health goals, and some partners identify health workforce as a key strategic focus in their policy and strategy documents. However, most do not identify it as a key focus, and few have a published specific policy or strategy to guide health workforce investments. Several partners include optional health workforce indicators in their monitoring and evaluation processes and/or require an impact assessment for issues such as the environment and gender equality. Very few, however, have embedded efforts in their governance mechanisms to strengthen health workforce assessments. On the other hand, most have participated in health workforce information exchange activities, including strengthening information systems and health labour market analyses. Although there is evidence of participation in efforts to strengthen health workforce assessments and (especially) information exchange, the achievement of this milestone of the Global Strategy requires more structured policies for the monitoring and evaluation of health workforce investments to optimize the value of these investments and contribute towards global and national health goals.

Knowledge of Internal Quality Control for Laboratory Tests among Laboratory Personnel Working in Department of Biochemistry in a Tertiary Care Center: A Descriptive Cross-sectional Study.

Introduction: Clinical laboratory holds a central position in patient care, thus, ensuring accurate laboratory test results is a necessity. Internal quality control ensures day-to-day laboratory consistency. However, unless practised, laboratory quality systems cannot be achieved. This depends on the efforts and commitment of laboratory personnel for its implementation. Hence, the aim of this study was to find out the knowledge of internal quality control for laboratory tests among laboratory personnel working in the Department of Biochemistry in a tertiary care centre. Methods: This was a descriptive cross-sectional study conducted from 1 July 2022 to 30 August 2022 after receiving ethical approval from Institutional Review Committee (Reference number: 2341/022). Semi-structured questionnaire was used to assess knowledge on internal quality control. Three non-respondents were excluded. The operational definition of the knowledge domain was set before finalizing the questionnaire. The convenience sampling method was used. Point estimate and 95% Confidence Interval were calculated. Results: Among 20 laboratory personnel, 5 (25%) (6.02-43.98, 95% Confidence Interval) had adequate knowledge of internal quality control. The mean knowledge score was 12±2.44. Conclusions: The prevalence of adequate knowledge of internal quality control for laboratory tests among laboratory personnel working in the Department of Biochemistry was similar to the other study done in a similar setting. Keywords: biochemistry; knowledge; laboratory personnel; quality control.

U.S. Cooperative Extension's response to substance misuse: A scoping review.

Background: The U.S. has experienced exponential growth in overdose fatalities over the past four decades and more than 22 million people are currently living with a substance use disorder (SUD). While great strides have been made in advancing the science of SUD prevention and treatment, proven programs and interventions are not commonly disseminated at scale in impacted communities. The U.S. Cooperative Extension System (Extension) has been recognized as a valued partner in addressing SUD in communities. Federal funding supporting Extension's response to the opioid epidemic reached $35 million in 2021 primarily through two grant programs: the United States Department of Agriculture's (USDA) Rural Health and Safety Education program; and the Substance Abuse and Mental Health Services Administration (SAMHSA) Rural Opioid Technical Assistance (ROTA) grants. The primary objective of this scoping review was to identify the range of Extension activities aimed at mediating substance misuse. Methods: Authors utilized the PRISMA-SCR model to complete this scoping review. Due to the nature of Extension work and the expectation that few activities would be cited in the peer-reviewed literature, the scoping review included a search of peer-reviewed databases, Extension websites for each state and U.S. territory, and the utilization of a web search engine. Upon initial analysis of records returned, authors noted a discrepancy between results returned and the number of states receiving ROTA grants. Thus, authors supplemented the PRISMA-SCR review protocol with a systematic procedure for investigating ROTA funded activities not readily apparent in the peer-reviewed or grey literature. Results: A total of 87 records met inclusion criteria. Findings included seven peer-reviewed articles and 80 results from the grey literature. An additional 11 ROTA grantees responded to requests for information regarding state level activities. Conclusions: Nationwide, Extension has scaled multiple efforts to address SUD operating through a loose confederation of organizations connected to the land-grant system. Most activities are funded by federal grants and focus on state-sponsored training and resource sharing. The volume of effort is significant, however, implementation at the community-level has been slow. Significant opportunities exist for local adoption of evidence-based practices aimed at mitigating SUD.

Evaluation of burnout and stress perception levels of Turkish dental laboratory technicians according to affecting factors during the COVID-19 pandemic.

BACKGROUND: The coronavirus disease-2019 (COVID-19) pandemic has caused healthcare professionals to face unequal acute workplace stress and burnout. This study aimed to analyze the potential impact of COVID-19 on the burnout and associated emotional stress conditions of Turkish dental technicians. METHODS: A 20-question demographic scale, Maslach Burnout Inventory (MBI), Sense of Coherence-13 (SoC-13), and Perceived Stress Scale-10 (PSS-10) were used to obtain data. A total of 152 participants answered these surveys directly and reported their stress burnout levels during the COVID-19 pandemic. RESULTS: Of all participants who agreed to participate in the survey, 39.5% were females and 60.5% were males. Regardless of demographic variables, the MBI-total (37.2 ± 11.71), SoC-13 total (53.81 ± 10.29), and PSS-10 total (21.25 ± 5.5) scores indicated moderate burnout, SoC, and perceived stress levels. According to sub-scores of the MBI; mean emotional exhaustion and depersonalization indicate low-level burnout, and mean personal accomplishment indicates moderate burnout. Long working hours increase burnout. No significant differences were observed according to demographic variables, except for work experience. A positive correlation was found between perceived stress and burnout. CONCLUSIONS: The findings showed that dental technicians working during the COVID-19 pandemic are influenced by emotional stress due to the outcomes of the pandemic. One reason for this situation might be the long working hours. Working arrangements, under-controlled disease risk factors, and lifestyle changes may improve stress levels.Key MessagesCOVID-19 outbreak exposed psychological returns to the general population, and especially to healthcare workers.Questionnaire method was applied to evaluate the burnout and stress levels among dental laboratory technicians during COVID-19 outbreak.Moderate levels of burnout and stress perception were detected. Long working time was one of the effective factors.

Automedicación en profesionales de un laboratorio farmacéutico paraguayo / Self-medication among professionals in a Paraguayan pharmaceutical company

Objetivo: determinar la prevalencia de la automedicación entre las personas trabajadoras de un laboratorio farmacéutico del área metropolitana de Asunción (Paraguay) y describir sus características.Método: estudio descriptivo transversal (nov 2016). Se incluyó a todas las personas adultas trabajadoras del laboratorio (N= 400). Se solicitó su participación por correo electrónico en dos ocasiones. Se midieron variables sociodemográficas, laborales y la automedicación mediante cuestionario heteroadministrado diseñado ad hoc y pilotado (síntomas que motivaron, frecuencia, grupo de fármaco, motivos, fuente de la recomendación, eficacia y peligro percibido, finalización, acción en caso de persistencia del síntoma, publicidad, lugar de compra, fuentes de información). Se realizó estadística descriptiva y bivariante.Resultados: participaron 166 personas (edad media 33,2 años; 60,2% hombres). El 34,3% se había automedicado en los tres meses anteriores. El 90,4% consideraba que la automedicación era peligrosa. La práctica de automedicación fue mayor en el nivel universitario que en el secundario (44,8% vs. 22,8%; p= 0,004). No se encontraron diferencias en función de la edad, el sexo, ni el tiempo trabajado en la empresa. La causa más frecuente fue el tratamiento del dolor de cabeza (64,9%) y los fármacos más consumidos, los analgésicos/antiinflamatorios (82,4%). El 66,6% no consideró necesario ir al médico para la prescripción y en el 35,1% de los casos fue el familiar o amigo quien lo recomendó.Conclusiones: la automedicación fue una práctica habitual en la población estudiada, aunque inferior a otros estudios. Es necesario el abordaje de este problema.(AU)

Objective: to determine the prevalence of self-medication among the persons working at a pharmaceutical company from the metropolitan area of Asunción (Paraguay), and to describe its characteristics.Method: a descriptive cross-sectional study (November 2016). All adult persons working at the company were included (N= 400). Their participation was requested by email in two occasions. Sociodemographic and occupational variables were measured, as well as self-medication, through a self-administered questionnaire, designed ad hoc and piloted (symptoms causing it, frequency, drug class, reasons, source of recommendation, efficacy and perceived danger, conclusion, action in case of symptom persistence, publicity, place of purchase, sources of information). Descriptive and bivariate statistics was conducted.Results: the study included 166 persons (mean age 33.2 years; 60.2% were male). Of these, 34.3% had self-medicated within the past three months; 90.4% considered that self-medication was dangerous. Self-medication was higher with university than with high school level (44.8% vs. 22.8%; p= 0.004). No differences were found based on age, gender or time worked at the company. The most frequent cause was treatment for headache (64.9%) and the drugs more widely used were analgesics / anti-inflammatories (82.4%); 66.6% of the participants did not consider it was necessary to see their doctor for prescription, and they followed recommendations by relatives or friends in 35.1% of cases.Conclusions: self-medication was a frequent practice among the population studied, although lower than in other studies. It is necessary to address this problem.(AU)

Microorganismos asociados a signos y síntomas en trabajadores de laboratorios en docencia e investigación / Microorganisms associated with signs and symptoms in laboratory workers in teaching and research

Introducción: La identificación de microorganismos de manipulación intencional, es primordial para el diseño de un proceso de vigilancia epidemiológica asociada al riesgo biológico en laboratorios. Objetivo: Determinar los microorganismos asociados con la presencia de signos y síntomas en trabajadores de laboratorios de una institución universitaria de una ciudad del Oriente Colombiano. Material y Métodos: Estudio descriptivo de corte transversal llevado a cabo en 33 laboratorios y 24 trabajadores. Resultados: se observó asociación de riesgo de presentar cefalea al manipular Oscillatoria spp p=0,09 y RR=2,0 (IC95% 1,29-3,1), faringitis con los hongos del género Aspergillus spp y Fusarium spp p≤0,05 RR=10,0 (IC95% 1,39-71,86) y rinitis alérgica en la manipulación de Staphylococcus aureus p≤0,05 y RR=2,3 (IC95% 1,05-5,17) Conclusión: Las infecciones adquiridas en los laboratorios son identificables si se mantienen los protocolos e información sobre los microorganismos de manipulación intencional y no intencional, siendo el inicio del proceso de vigilancia epidemiológica asociada al riesgo biológico. (AU)

Introduction: The identification of microorganisms of intentional manipulation is essential for the design of an epidemiological surveillance process associated with biological risk in laboratories. Objective: To determine the microorganisms associated with the presence of signs and symptoms in laboratory workers of a university institution in a city of eastern Colombia. Material and Methods: Descriptive cross-sectional study carried out in 33 laboratories and 24 workers. Results: risk association of presenting headache when handling Oscillatoria spp p=0.09 and RR=2.0 (IC95% 1.29-3.1), pharyngitis with fungi of the genus Aspergillus spp and Fusarium spp p≤0.05 RR=10.0 (IC95% 1.39-71.86) and allergic rhinitis when handling Staphylococcus aureus p≤0.05 and RR=2.3 (IC95% 1.05-5.17) Conclusions: Laboratory-acquired infections are identifiable if protocols and information on intentional and unintentional handling microorganisms are maintained, being the beginning of the epidemiological surveillance process associated with biological risk. (AU)

Documento técnico: Manual de procedimientos para el diagnóstico bacteriológico de la tuberculosis mediante cultivos / Technical document: Manual of procedures for the bacteriological diagnosis of tuberculosis through cultures

El documento contiene las directrices para estandarizar y proporcionar al personal de laboratorio los procedimientos técnicos del cultivo para el diagnóstico de la tuberculosis

A Multilingual Tool for Standardized Laboratory Biosafety and Biosecurity Assessment and Monitoring.

Control of infectious diseases requires the handling of infectious materials by both clinical and public health laboratories with exposure risks for laboratory personnel and environment. A comprehensive tool for assessing the capacity to manage these risks could enable the development of action plans for mitigation. Under the framework of the Global Health Security Agenda action package for biosafety and biosecurity, the authors developed a tool dedicated to assessing laboratory biosafety and biosecurity. The Biosafety and Biosecurity Laboratory Assessment Tool (BSS LAT) assesses the status of all laboratory biosafety core requirements across 10 different modules. It consists of a standardized spreadsheet-based tool that provides automatic scoring. It is designed to support national, regional, and global efforts to strengthen biosafety in clinical, public health, and veterinary laboratories. The BSS LAT was first used in Burkina Faso in collaboration with the African Society for Laboratory Medicine and the US Centers for Disease Control and Prevention to support the country in strengthening their biorisk management system. Since then, it has been successfully used in other countries (ie, Armenia, Burundi, Cameroon, Ghana, Guinea, Kazakhstan, Liberia), various settings (medical and veterinary laboratories), and translated into several languages (eg, English, French, Russian). The BSS LAT is a multipurpose tool that assists with standardization of biosafety and biosecurity requirements for all laboratories working with infectious materials, serves as a self-assessment guide for laboratories to develop improvement plans and reinforce capacities, and serves as a training guide for individual laboratories and networks or at the national level. The BSS LAT can also be used as a monitoring tool for the assessment of biosafety and biosecurity across all laboratories working with infectious materials at the national, regional, and global levels.

Documento Técnico: Manual de pruebas moleculares para el diagnóstico bacteriológico y de sensibilidad de la tuberculosis / Technical Document: Manual of molecular tests for the bacteriological and susceptibility diagnosis of tuberculosis

El documento contiene las directrices estandarizadas a fin de proporcionar al personal de laboratorio los procedimientos técnicos de las pruebas moleculares para el diagnóstico bacteriológico y de la sensibilidad de la tuberculosis.

Impactful scientists have higher tendency to involve collaborators in new topics.

In scientific research, collaboration is one of the most effective ways to take advantage of new ideas, skills, and resources and for performing interdisciplinary research. Although collaboration networks have been intensively studied, the question of how individual scientists choose collaborators to study a new research topic remains almost unexplored. Here, we investigate the statistics and mechanisms of collaborations of individual scientists along their careers, revealing that, in general, collaborators are involved in significantly fewer topics than expected from a controlled surrogate. In particular, we find that highly productive scientists tend to have a higher fraction of single-topic collaborators, while highly cited-i.e., impactful-scientists have a higher fraction of multitopic collaborators. We also suggest a plausible mechanism for this distinction. Moreover, we investigate the cases where scientists involve existing collaborators in a new topic. We find that, compared to productive scientists, impactful scientists show strong preference of collaboration with high-impact scientists on a new topic. Finally, we validate our findings by investigating active scientists in different years and across different disciplines.

Documento Técnico: Manual de Procedimientos de la Baciloscopia para el Diagnóstico Bacterológico de la Tuberculosis / Technical Document: Manual of Bacilloscopy Procedures for the Bacterological Diagnosis of Tuberculosis

El documento proporciona al personal de laboratorio los procedimientos estandarizados técnicos de la baciloscopia para el diagnóstico bacteriológico de la TB

Fiocruz, Ministério da Saúde do Brasil e OPAS capacitam técnicos de laboratório de 7 países da América Latina em diagnóstico de varíola dos macacos

A Fundação Oswaldo Cruz (Fiocruz), o Ministério da Saúde do Brasil e a Organização Pan-Americana da Saúde (OPAS) realizam nesta quinta e sexta-feira (10), na cidade do Rio de Janeiro, Brasil, um treinamento sobre diagnóstico e detecção laboratorial da varíola dos macacos, com a participação de especialistas de sete países da América Latina.

Core competencies for a biomedical laboratory scientist - a Delphi study.

BACKGROUND: After completing university education, biomedical laboratory scientists work in clinical laboratories, in biomedical research laboratories, in biotech, and in pharmaceutical companies. Laboratory diagnostics have undergone rapid development over the recent years, with the pace showing no signs of abatement. This rapid development challenges the competence of the staff and will most certainly influence the education of future staff. This study aimed to examine what was considered the necessary competencies needed to pursue a career as a biomedical laboratory scientist. METHODS: A modified Delphi technique was used, with the panel of experts expressing their views in a series of three questionnaire. Consensus was defined as the point which 75 % or more of the panel participants agreed that a particular competency was necessary. RESULTS: The study highlights the perceived importance of mostly generic competencies that relate to quality, quality assurance, and accuracy, as well as different aspects of safety, respect, trustworthiness (towards patients/clients and colleagues), and communication skills. The results also stress the significance of self-awareness and professionality. CONCLUSIONS: We identified important competencies for biomedical laboratory scientists. Together with complementary information from other sources, i.e., guidelines, laws, and scientific publications, the competencies identified can be used as learning outcomes in a competency-based education to provide students with all the competencies needed to work as professional biomedical laboratory scientists.

Monkeypox outbreak questions intensify as cases soar.

Rapid emergence of hundreds of cases around the world alarms public health officials and scientists.

Beyond inclusion: Enacting team equity in precision medicine research.

PURPOSE: To identify meanings of and challenges to enacting equitable diversification of genomics research, and specifically precision medicine research (PMR), teams. METHODS: We conducted in-depth interviews with 102 individuals involved in three U.S.-based precision medicine research consortia and conducted over 400 observation hours of their working group meetings, consortium-wide meetings, and conference presentations. We also reviewed published reports on genomic workforce diversity (WFD), particularly those relevant to the PMR community. RESULTS: Our study finds that many PMR teams encounter challenges as they strive to achieve equitable diversification on scientific teams. Interviewees articulated that underrepresented team members were often hired to increase the study's capacity to recruit diverse research participants, but are limited to on-the-ground staff positions with little influence over study design. We find existing hierarchies and power structures in the academic research ecosystem compound challenges for equitable diversification. CONCLUSION: Our results suggest that meaningful diversification of PMR teams will only be possible when team equity is prioritized as a core value in academic research communities.

Feeling like a 'real' scientist.

Peer reviewing helped a graduate student to finally gain a sense of belonging within the research community.